Determination of progesterone concentration during the estrous cycle in dairy cows using a chemiluminescence assay

Autores

  • Luiz Ernandes Kozicki Pontifícia Universidade Católica do Paraná
  • Saulo Henrique Weber Pontifícia Universidade Católica do Paraná
  • Louise Helene Bacher Pontifícia Universidade Católica do Paraná
  • Isabella Sellmer Ramos Pontifícia Universidade Católica do Paraná
  • Francisco Romano Gaievski Pontifícia Universidade Católica do Paraná
  • Marcos Vinicius Schaus Pontifícia Universidade Católica do Paraná
  • Rafaela Talini Pontifícia Universidade Católica do Paraná
  • Romildo Romualdo Weiss Universidade Federal do Paraná
  • Tácia Bergstein-Galan Universidade Federal do Paraná
  • Márcio Sporski Segui Pontifícia Universidade Católica do Paraná
  • Carlos Alberto Mayora Aita Pontifícia Universidade Católica do Paraná

DOI:

https://doi.org/10.7213/1981-4178.2018.16006

Palavras-chave:

Immunochemiluminescence, Chemiluminescence assay (CLIA), Progesterone, Dairy cows, Hormonal profile, Physiological estrus cycle.

Resumo

The study aimed to determine the patterns of serum progesterone concentration in estrous cycle in dairy cows by a chemiluminescence assay (CLIA). Four non-lactating multiparous Jersey cows were used. Animals with a corpus luteum (CL) in any of the ovaries were induced into estrus. Day zero (d0) of the estrous cycle was defined as the day of visible estrus. Blood samples were collected and ultrasonography (US) of the ovaries were performed until a new manifestation of visible estrus was observed. The lengths of the estrous cycles (estrus to estrus) of the four cows were 20, 21, 22, and 23 days. The mean serum concentrations of P4 (x ± s) were 2.8 ± 1.4 ng/mL in proestrus, 2.4 ± 1.5 ng/mL in estrus, 2.0 ± 1.8 ng/mL in metestrus, and 11.9 ± 5.7 ng/mL in diestrus. The follicular and luteal phases of the estrous cycle were established based on P4 concentrations. P4 serum concentrations ≥5.48 ng/mL indicated the presence of functional CL, which was observed from d3 to d12 of the cycle. P4 concentrations decreased from d13 until next estrus. Thus, the previously mentioned P4 serum concentration was established as the limit for a predominantly functional CL. P4 concentrations < 5.48 ng/mL indicated a nonfunctional CL or CL that is not yet fully formed, which is observed in metestrus. A P4 standard curve, constructed based on the Lorentz distribution, was used to determine values of < 5.48 ng/mL of serum P4 concentration for the follicular phase and ≥ 5.48 ng/mL for the luteal phase. Data obtained from US examinations were consistent with P4 concentrations determined using CLIA. In conclusion, the automated CLIA was efficient in determining the P4 concentrations during the various stages of estrous cycles in dairy cows. The findings of the study will help researchers in the animal reproduction field. We recommend the use of CLIA because it´s available in many laboratories worldwide with the ability to process thousands of samples per day.

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Publicado

05-06-2018

Como Citar

1.
Kozicki LE, Weber SH, Bacher LH, Ramos IS, Gaievski FR, Schaus MV, Talini R, Weiss RR, Bergstein-Galan T, Segui MS, Mayora Aita CA. Determination of progesterone concentration during the estrous cycle in dairy cows using a chemiluminescence assay. Rev. Acad. Ciênc. Anim. [Internet]. 5º de junho de 2018 [citado 3º de novembro de 2024];16:1-11. Disponível em: https://periodicos.pucpr.br/cienciaanimal/article/view/e16006

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