CLONAL DIVERSITY OF STREPTOCOCCUS MUTANS CLARKE (1924) IN CARIES-FREE ADULTS

Autores

  • Rosimeire Takaki Rosa
  • Marcelo Henrique Napimoga
  • José Francisco Höfling
  • Reginaldo Bruno Gonçalves
  • Edvaldo Antonio Ribeiro Rosa

DOI:

https://doi.org/10.7213/reb.v27i58.22705

Palavras-chave:

Streptococcus mutans, Dental caries, MLEE, Clonal diversity, Isoenzymes

Resumo

Very few information about the clonal profile of Streptococcus mutans in caries-free adults is available. Microbial samples of saliva, tongue dorsum, and dental biofilm were taken from eight caries-free volunteers (DMFT = 0). Presumptive colonies of mutans streptococci were identified until species level. After confirmation, S. mutans strains were grown in liquid culture media and harvested for whole-cell protein extraction. Multilocus Enzyme Electrophoresis (MLEE) separated protein extracts and the bands’ patterns were assessed for six enzymes. Results showed that two or more distinct clonal types (avg = 4.75) were found cocolonizing all the enrolled subjects, confirming the premise that caries-free subjects are prone to be colonized by more than one strain of S. mutans.

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Publicado

2005-11-24

Como Citar

Rosa, R. T., Napimoga, M. H., Höfling, J. F., Gonçalves, R. B., & Rosa, E. A. R. (2005). CLONAL DIVERSITY OF STREPTOCOCCUS MUTANS CLARKE (1924) IN CARIES-FREE ADULTS. Estudos De Biologia, 27(58). https://doi.org/10.7213/reb.v27i58.22705

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