TYPING STREPTOCOCCUS MUTANS BY MULTILOCUS ENZYME ELECTROPHORESIS

Authors

  • Rosimeire Takaki Rosa
  • Marcelo Henrique Napimoga
  • José Francisco Höfling
  • Reginaldo Bruno Gonçalves
  • Edvaldo Antonio Ribeiro Rosa

DOI:

https://doi.org/10.7213/aor.v1i3.22873

Keywords:

Streptococcus mutans, Dental caries, Isoenzymes

Abstract

OBJECTIVES: It was evaluated the use of multilocus enzyme electrophoresis (MLEE) for the characterization of Streptococcus mutans. MATERIAL AND METHODS: Five strains of such microrganisms were grown in liquid culture medium and their cytoplasm proteins were extracted. After gel electrophoreses, the catalytic activity was explored for 23 isoenzymes. RESULTS: Six enzymes (mannitol-1-phosphate dehydrogenase, mannose-phosphate isomerase, nucleoside phosphorylase, phenylalanyl-leucine peptidase, leucine aminopeptidase, and glutamic-oxalacetic transaminase) furnished bands that allowed the strains characterization at intra-specific level. CONCLUSION: MLEE is a robust technique that may be used to explore diversity among clinical isolates of cariogenic S. mutans in epidemiological surveys.

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Published

2005-11-27

How to Cite

Rosa, R. T., Napimoga, M. H., Höfling, J. F., Gonçalves, R. B., & Ribeiro Rosa, E. A. (2005). TYPING STREPTOCOCCUS MUTANS BY MULTILOCUS ENZYME ELECTROPHORESIS. Archives of Oral Research, 1(3). https://doi.org/10.7213/aor.v1i3.22873

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Articles